Detection of single methylated cytosine using junction-forming DNA probes.
نویسندگان
چکیده
DNA methylation is an epigenetic mechanism for transcriptional regulation. The methylation process controls cellular differentiation and is defective in many diseases including cancer. Therefore, the development of a simple method for analysing cytosine methylation in a target gene is required. Here we report a conceptually new method for sequence-selective chemical modification of a single cytosine in single-stranded DNA (ssDNA) using two DNA probes to form a DNA three-way junction with the ssDNA. The method was successfully used in a simple quantitative polymerase-chain-reaction-based assay for discrimination of a single methylated cytosine.
منابع مشابه
Sequence-selective modification of DNA cytosine by using junction-forming DNA probes and its application to the detection of single cytosine methylation.
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عنوان ژورنال:
- The Analyst
دوره 139 9 شماره
صفحات -
تاریخ انتشار 2014